University of Tsukuba, Laboratory of Biology for Extreme Molecule

主な研究テーマ

• RNAウイルスの生態

  ・糸状菌RNAウイルスの生態と機能の解明
  ・多様なRNAウイルスの探索
  ・網羅的RNAウイルス検出技術の開発

• RNAプラスミドの生態

  ・糸状菌RNAプラスミドの生態と機能の解明
  ・多様なRNAプラスミドの探索
  ・網羅的RNAプラスミド検出技術の開発

• 細胞外膜小胞（メンブレンベシクル）の研究

  ・糸状菌のメンブレンベシクル産生機構の解明
  ・RNAウイルスやRNAプラスミドのベクターとしての機能解析
  ・発酵食品中の糸状菌由来メンブレンベシクルの研究

連絡先

E-mail　:　urayama.shunichi.gn(at)u.tsukuba.ac.jp
　　　　※(at)を@にかえてください。

学歴

2009年～2012年　東京農工大学大学院　連合農学研究院
2009年～2007年　東京農工大学大学院　生物制御科学専攻
2003年～2007年　東京農工大学農学部　応用生物科学科
2000年～2003年　都立戸山高校

職歴

2017年～　現職
2014年～2017年　海洋研究開発機構　ポストドクトラル研究員
2013年～2014年　東京農工大学　産学官特別研究員
2011年～2013年　日本学術振興会特別研究員（DC2）

受賞歴

1. 日本微生物学連盟　野本賞　「RNAウイルスの生態学的研究」. 2025年.
2. 第１０回　日本微生物生態学会奨励賞　「微生物に潜むRNAウイルスの生態学的研究」. 2024年.
3. オープンイノベーションによるウイルス二本鎖RNA精製技術の実用化.
   海洋研究開発機構　研究開発功績賞. 2019年4月.
4. 2017年度 M&E論文賞. 日本微生物生態学会. 2017年8月.
5. Excellent Poster Prize. The 2nd Korea-Japan Joint Symposium 2012, March 27-28, 2012.
6. 日本植物病理学会学生優秀発表賞. 平成24年度植物病理学会大会. 2012年3月.

研究業績

1. Akihiro Ninomiya, Keisuke Masuda, Tsuyoshi Yamada, Misa Kuroki, Sayaka Ban, Takashi Yaguchi, Urayama S, Daisuke Hagiwara. Rediscovery of viomellein as an antibacterial compound and identification of its biosynthetic gene cluster in dermatophytes. Applied and Environmental Microbiology, e02431-24 (2025)
   
   
2. Nesma M Samir, Jacob Locke-Gotel, Urayama S, Adel A El-Morsi, Ghada A El-Sherbeny, Yongju Huang, Bruce DL Fitt, Hiromitsu Moriyama, Robert HA Coutts, Ioly Kotta-Loizou*. Molecular characterization of a polymycovirus in Leptosphaeria biglobosa. Archives of Virology, 170(4), 66 (2025)
   
   
3. Buma S, Urayama S, Suo R, Itoi S, Okada S, Ninomiya A. Mycoviruses from Aspergillus fungi involved in fermentation of dried bonito. Virus Research, 350: 199470 (2024)
   
   
4. Wasiatus Sa'diyah, Zhao Y-J, Chiba Y, Kondo H, Suzuki N, Ban S, Yaguchi T, Urayama S, Hagiwara D. New lineages of RNA viruses from clinical isolates of Rhizopus microsporus revealed by fragmented and primer-ligated dsRNA sequencing (FLDS) analysis. mSphere, 9: e00345-24 (2024)
   
   
5. Sidra Hassan, Urayama S, Saba Shabeer, Tahseen Ali Kiran, Chien-Fu W, Moriyama H, Coutts R, Kotta-Loizou I, Jamal A. Molecular and biological characterization of a novel partitivirus from Talaromyces pinophilu. Virus Research, 343: 199351 (2024)
   
   
6. Urayama S*, Zhao Y-J, Kuroki M, Chiba Y, Ninomiya A, Hagiwara D. Greetings from virologists to mycologists: A review outlining viruses that live in fungi. Mycoscience, 65: 1-11 (2024)
   
   
7. Urayama S*, Fukudome, AHirai M, Okumura T, Nishimura Y, Takaki Y, Kurosawa N, E. V. Koonin, Krupovic M, Nunoura T. Double-stranded RNA sequencing reveals distinct riboviruses associated with thermoacidophilic bacteria from hot springs in Japan. Nature Microbiology https://doi.org/10.1038/s41564-023-01579-5 (2024)
   ☆読売新聞記事☆
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8. Takahashi M, Masuda Y, Chiba Y, Urayama S, Nagasaki K. DsRNA sequencing revealed a previously missed terminal sequence of a+ ssRNA virus that infects dinoflagellate Heterocapsa circularisquama. Virus Genes, 60: 97–99 (2024)
   
   
9. Zhao, Y-J*, Hosoya T, Urayama S, Hagiwara D*. Seven new mycoviruses identified from isolated ascomycetous macrofungi. Virus Research 339: 199290 (2024)
   
   
10. Suzuki, K., Urayama, S., Hirai, Y., Yamaguchi, T., Ujiie, K., Akiyama, H. Designing Educational Board Games of Global Anthropogenic Issues through Interdisciplinary Collaboration. Proceedings of 26th International Conference on Interactive Collaborative Learning (ICL2023), 259-262, Madrid, Spain (2023)
    
    
11. Hassan S, Urayama S, Saba S, Chien-Fu W, Moriyama H, Coutts R, Kotta-Loizou I, Jamal A. Molecular and biological characterization of a partitivirus from Paecilomyces variotii. Journal of General Virology 104(11): 001925 (2023)
    
    
12. Kuroki M, Yaguchi T, Urayama S, Hagiwara D*. Experimental verification of strain-dependent relationship between mycovirus and its fungal host. iScience 26: 8 (2023)
    
    
13. Zhao Y-J, Shirouzu T, Chiba Y, Hosaka K, Moriyama H, Urayama SI*, Hagiwara D. Identification of novel RNA mycoviruses from wild mushroom isolates in Japan. Virus Research, 325: 199045 (2023)
    
    
14. Chiba Y, Yabuki A, Takaki Y, Nunoura T, Urayama S*, Hagiwara D*. The first identification of a narnavirus in Bigyra, a marine-protist. Microbes and Environments, 38: ME22077 (2023)
    
15. Urayama S*, Takaki Y, Chiba Y, Zhao Y, Kuroki M, Hagiwara D, Nunoura T. Eukaryotic Microbial RNA Viruses—Acute or Persistent? Insights into Their Function in the Aquatic Ecosystem. Microbes and Environments, 37: ME22034 (2022) doi: 10.1264/jsme2.ME22034
    
16. Ikeda A, Chiba Y, Kuroki M, Urayama S*, Hagiwara D*. Efficient elimination of RNA mycoviruses in Aspergillus species using RdRp-inhibitors ribavirin and 2'-C-methylribonucleoside derivatives. Frontiers in Microbiology, 13:1024933 (2022)
    
    
17. Sakaguchi S, Urayama S, Takaki Y, Wu H, Suzuki Y, Nunoura T, Nakano T, Nakagawa S*. NeoRdRp: A comprehensive dataset for identifying RNA-dependent RNA polymerase of various RNA viruses from metatranscriptomic data. Microbes and Environments, 37: ME22001 (2022)
    
    
18. Mizutani Y, Chiba Y, Urayama S, Tomaru Y, Hagiwara D, Kimura K*. Detection and Characterization of RNA Viruses in Red Macroalgae (Bangiaceae) and Their Food Product (Nori Sheets). Microbes and Environments 37.2 (2022): ME21084.
    
    
19. Oiki S, Nasuno R, Urayama S, Takagi H, Hagiwara D*. Intracellular production of reactive oxygen species and a DAF-FM-related compound in Aspergillus fumigatus in response to antifungal agent exposure. Scientific Reports, 12: 13516 (2022)
    
    
20. Ninomiya A, Urayama S, Hagiwara D*. Antibacterial diphenyl ether production induced by co-culture of Aspergillus nidulans and Aspergillus fumigatus. Applied Microbiology and Biotechnology, 106: 4169–4185 (2022)
    
    
21. Kadoya S, Urayama S, Nunoura T, Hirai M, Takaki Y, Kitajima M, Nakagomi T, Nakagomi O, Okabe S, Nishimura O, Sano D*. The Intrapopulation Genetic Diversity of RNA Virus may Influence the Sensitivity of Chlorine Disinfection. Frontiers in Microbiology, 13: 839513 (2022)
    
    
22. Takahashi M, Wada K, Urayama S, Masuda Y, Nagasaki K*. Degenerate PCR Targeting the Major Capsid Protein Gene of HcRNAV and Related Viruses. Microbes and Environments, 37.5: ME21075 (2022)
    
    
23. Hirai J*, Urayama S, Takaki Y, Hirai M, Nagasaki K, Nunoura T. RNA virosphere in a marine zooplankton community in the subtropical western North Pacific. Microbes and Environments, 37.5: ME21066 (2022)
    
    
24. Oiki S, Yaguchi T, Urayama S, Hagiwara D*. Wide distribution of resistance to the fungicides fludioxonil and iprodione in Penicillium species. PLoS One, 17.1: e0262521 (2022)
    
    
25. Chiba Y, Oiki S, Zhao Y-J, Nagano Y, Urayama S*, Hagiwara D*. Splitting of RNA-dependent RNA Polymerase is Common in Narnaviridae: Identification of a type II Divided-RdRp from Deep-Sea Fungal Isolates. Virus Evol. 7(2), veab095 (2021)
    
    
26. Takahashi H, Oiki S, Kusuya Y, Urayama S, Hagiwara D*. Intimate genetic relationships and fungicide resistance in multiple strains of Aspergillus fumigatus isolated from a plant bulb. Environmental Microbiology, 23: 5621-5638 (2021)（プレスリリース）
    
    
27. Hirai M, Takaki Y*, Kondo F, Horie M, Urayama S, Nunoura T. RNA viral metagenome analysis from subnanogram dsRNA using fragmented and primer ligated dsRNA sequencing (FLDS). Microbes and Environments, 36: ME20152 (2021).
    
    
28. Izumi T, Morioka Y, Urayama, S, Motooka D, Tamura T, Kawagishi T, ... & Fukuhara T. DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples. Viruses, 13(7), 1310. (2021).
    
    
29. Uehara-Ichiki T*, Urayama S, Hirai M, Takaki Y, Nunoura T, Fujinaga M, Hanada K. Complete genome sequence of Sikte (Sitke) waterborne virus, a member of the genus Tombusvirus. Archives of Virology, 166: 991-994 (2021)
    
    
30. Fujita R*, Inoue M, Takamatsu T, Arai H, Nishino M, Abe N, Itokawa K, Nakai M, Urayama S, Chiba Y, Amoa-Bosompem M, Kunimi Y. Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae. Frontiers in microbiology, 11: 3558 (2021)
    
    
31. Takahashi H, Umemura M, Ninomiya A, Kusuya Y, Shimizu M, Urayama S, Watanabe A, Kamei K, Yaguchi T, Hagiwara D*. Interspecies Genomic Variation and Transcriptional Activeness of Secondary Metabolism-Related Genes in Aspergillus Section Fumigati. Frontiers in Fungal Biology, 2: 656751. doi: 10.3389/ffunb.2021.656751 (2021)
    
    
32. Chiba Y, Oiki S, Yaguchi T, Urayama S*, Hagiwara D*. Discovery of divided RdRp sequences and a hitherto unknown genomic complexity in fungal viruses. Virus Evol. 7(1), veaa101 (2020) （プレスリリース）
    
    
33. Takahashi-Nakaguchi A* , Shishido E, Yahara M, Urayama S, Ninomiya A, Chiba Y, Sakai K, Hagiwara D, Chibana H, Moriyama H, Gonoi T. Phenotypic and molecular biological analysis of polymycovirus AfuPmV-1M from Aspergillus fumigatus: Reduced fungal virulence in a mouse infection model. Front. Microbiol. 11: 607795 (2020)
    
    
34. Urayama S*, Doi N, Kondo F, Chiba Y, Takaki Y, Hirai M, Minegishi Y, Hagiwara D, Nunoura T. Diverged and active partitiviruses in lichen. Front. Microbiol. 11: 561344 (2020)
    
    
35. Ninomiya A, Urayama S, Suo R, Itoi S, Fuji S, Moriyama H, Hagiwara D*. Mycovirus-induced tenuazonic acid production in a rice blast fungus Magnaporthe oryzae. Front. Microbiol. 11: 1641 (2020)
    
    
36. Chiba Y, Tomaru Y, Shimabukuro H, Kimura K, Hirai M, Takaki Y, Hagiwara D, Nunoura T, Urayama S *. Viral RNA genomes identified from marine macroalgae and a diatom. Microbes and Environments, 35(3): ME10016 (2020)
    
    
37. Kadoya S, Urayama S, Nunoura T, Hirai M, Takaki Y, Kitajima M, Nakagomi T, Nakagomi O, Okabe S, Nishimura O, Sano D. Bottleneck Size-Dependent Changes in the Genetic Diversity and Specific Growth Rate of a Rotavirus A Strain. Journal of Virology 94:e02083-19 (2020)
    
    
38. Urayama S*, Takaki Y, Hagiwara D, Nunoura T. dsRNA-seq reveals novel RNA virus and virus-like complete genome sequences from Hymeniacidon sp. sponge. Microbes and Environments. 35(2), ME19132 (2020)
    
    
39. Takahashi-Nakaguchi A, Shishido E, Yahara M, Urayama S, Sakai K, Chibana H, Kamei K, Moriyama H, Gonoi T. Analysis of an intrinsic mycovirus associated with reduced virulence of the human pathogenic fungus Aspergillus fumigatus. Frontiers in Microbiology, 10:3045. doi: 10.3389/fmicb.2019.03045. (2020)
    
    
40. Fukasawa F, Hirai M, Takaki Y, Shimane Y, Cathleen E Thomas, Urayama S, Nunoura T, Koyama S. A new polycipivirus identified in Colobopsis shohki. Archives of Virology. 165(3), 761-763 (2020)
    
    
41. Higashiura T, Kato Y, Urayama S, Hayashi O, Aihara M, Fukuhara T, Fuji S, Kobayashi T, Hase S, Arie T, Teraoka T, Komatsu K, Moriyama H. Magnaporthe oryzae chrysovirus 1 strain D confers growth inhibition to the host fungus and exhibits multiform viral structural proteins. Virology, 535:241-254 (2019)
    
    
42. Urayama S*, Takaki Y, Nishi S, Yoshida-Takashima Y, Deguchi S, Takai K, Nunoura T. Unveiling the RNA virosphere associated with marine microorganisms. Molecular Ecology Resources, 18:1444-1455, (2018)
    (Open Access)
    JAMSTECニュースリリース
    
    
43. Urayama S*, Takaki Y, Nunoura T, Miyamoto N. Complete genome sequence of a novel RNA virus identified from a deep-sea animal, Osedax japonicus. Microbes and Environments. 33(4):446-449 (2018)
    
    
44. Okada R, Ichinose S, Takeshita K, Urayama S. I, Fukuhara T, Komatsu K, Arie T, Ishihara A, Egusa M, Kodama M, Moriyama H. Molecular characterization of a novel mycovirus in Alternaria alternata manifesting two-sided effects: Down-regulation of host growth and up-regulation of host plant pathogenicity. Virology, 519: 23-32 (2018).
    
    
45. Aihara M, Urayama S, Le MT, Katoh Y, Higashiura T, Fukuhara T, Arie T, Teraoka T, Komatsu K, Moriyama H. Infection by Magnaporthe oryzae chrysovirus 1 strain A triggers reduced virulence and pathogenic race conversion of its host fungus, Magnaporthe oryzae. Journal of General Plant Pathology, 84(2): 92-103 (2018).
    
    
46. Yoshida M, Mochizuki T, Urayama S, Yoshida-Takashima Y, Nishi S, Hirai M, Nomaki H, Takaki Y, Nunoura T, Takai K. Quantitative viral community DNA analysis reveals the dominance of single-stranded DNA viruses in offshore upper bathyal sediment from Tohoku, Japan. Frontiers in Microbiology, doi: 10.3389/fmicb.2018.00075, (2018)
    
    
47. Urayama S, Kimura Y, Katoh Y, Ohta T, Onozuka N, Fukuhara T, Arie T, Teraoka T, Komatsu K, Moriyama H. Suppressive effects of mycoviral proteins encoded by Magnaporthe oryzae chrysovirus 1 strain A on conidial germination of the rice blast fungus. Virus Research, 223(2): 10–19 (2016)
    
    
48. Koyama S, Sakai C, Cathleen E T, Nunoura T, Urayama S. A new member of the family Totiviridae associated with arboreal ants (Camponotus nipponicus). Archives of virology, 161(7): 2043–2045 (2016)
    
    
49. Urayama S*, Takaki Y, Nunoura T. FLDS: A comprehensive dsRNA sequencing method for intracellular RNA virus surveillance. Microbes and Environments, 31(1): 33–40 (2016)
    
    
50. Komatsu K, Urayama S, Katoh Y, Fuji S, Hase S, Fukuhara T, Arie T, Teraoka T, Moriyama H. Detection of Magnaporthe oryzae chrysovirus 1 in Japan and establishment of a rapid, sensitive and direct diagnostic method based on reverse transcription loop-mediated isothermal amplification. Archives of virology, 161(2):317–326 (2016)
    
    
51. Koyama S, Urayama S, Ohmatsu T, Sassa Y, Sakai C, Takata M, Hayashi S, Nagai M, Furuya T, Moriyama H, Satoh T, Ono S, Mizutani T. Identification, characterization and full-length sequence analysis of a novel dsRNA virus isolated from the arboreal ant Camponotus yamaokai. Journal of General Virology, 96:1930–1937 (2015)
    
    
52. Urayama S*, Yoshida-Takashima Y, Yoshida M, Tomaru Y, Moriyama H, Takai K, Nunoura T. A new fractionation and recovery method of viral genomes based on nucleic acid composition and structure using tandem column chromatography. Microbes and Environments, 30(2):199–203 (2015)
    
    
53. Urayama S, Katoh Y, Fukuhara T, Arie T, Moriyama H, Teraoka T. Rapid detection of Magnaporthe oryzae chrysovirus 1-A from fungal colonies on agar plates and lesions of rice blast. Journal of General Plant Pathology, 1-6 (2014)
    
    
54. Urayama S, Fukuhara T, Moriyama H, Toh-e A, Kawamoto S. Heterologous expression of a gene of Magnaporthe oryzae chrysovirus 1 strain A disrupts growth of the human pathogenic fungus Cryptococcus neoformans. Microbiology and Immunology, 58(5):294–302 (2014)
    
    
55. Urayama S, Sakoda H, Takai R, Katoh Y, Tuong M L, Fukuhara T, Arie T, Teraoka T, Moriyama H. A dsRNA mycovirus, Magnaporthe oryzae chrysovirus 1-B, suppresses vegetative growth and development of the rice blast fungus. Virology, 448: 265–273 (2014)
    
    
56. Urayama S, Ohta T, Onozuka N, Sakoda H, Fukuhara T, Arie T, Teraoka T, Moriyama H. Characterization of Magnaporthe oryzae chrysovirus 1 structural proteins and their expression in Saccharomyces cerevisiae. Journal of Virology, 86(15):8287–8295 (2012)
    
    
57. Fukuhara T, Urayama S, Okada R, Kiyota E, Moriyama H. Detection of long and short double-stranded RNAs. Methods Mol. Biol. 744:129-144 (2011)
    
    
58. Urayama S, Kato S, Suzuki Y, Aoki N, Minh T L, Arie T, Teraoka T, Fukuhara T, Moriyama H. Mycoviruses related to chrysovirus affect vegetative growth in the rice blast fungus Magnaporthe oryzae. Journal of General Virology, 91(12):3085–3094 (2010)
    
    
59. Urayama S, Moriyama H, Aoki N, Nakazawa Y, Okada R, Kiyota E, Miki D, Shimamoto K, Fukuhara T. Knock-down of OsDCL2 in rice negatively affects maintenance of the endogenous dsRNA virus, Oryza sativa endornavirus. Plant and Cell Physiology, 51(1):58–67 (2009)
    
    （ * 責任著者）